The proposed project aims at developing an easy, ideally tissue culture-independent, RNA virus-based CRISPR/Cas-system to enable targeted and heritable genome modifications in potato. Following a first proof-of-concept phase, in which PDS expression will be targeted, potato genes, which will allow to grow potato plants under unfavorable climatic conditions, will be edited. The major goals, gene edited potato plants and tissue-culture free systems, will require the development of a novel, innovative transformation method.